An essential bacterial-type cardiolipin synthase mediates cardiolipin formation in a eukaryote

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An essential bacterial-type cardiolipin synthase mediates cardiolipin formation in a eukaryote.

Cardiolipin is important for bacterial and mitochondrial stability and function. The final step in cardiolipin biosynthesis is catalyzed by cardiolipin synthase and differs mechanistically between prokaryotes and eukaryotes. To study the importance of cardiolipin synthesis for mitochondrial integrity, membrane protein complex formation, and cell proliferation in the human and animal pathogenic ...

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An unusual phosphatidylethanolamine-utilizing cardiolipin synthase is discovered in bacteria.

C ardiolipin (also known as diphosphatidylglycerol) is a four-chained anionic membrane phospholipid composed of two phosphatidyl moieties joined by a glycerol link (Fig. 1). It was first isolated from beef heart as a serologically active phospholipid (1), hence the name cardiolipin. In animals, plants, and lower eukaryotic organisms (e.g., yeast), cardiolipin is a component of the inner mitocho...

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Functional Specificity of Cardiolipin Synthase Revealed by the Identification of a Cardiolipin Synthase CrCLS1 in Chlamydomonas reinhardtii

Phosphatidylglycerol (PG) and cardiolipin (CL) are two essential classes of phospholipid in plants and algae. Phosphatidylglycerophosphate synthase (PGPS) and cardiolipin synthase (CLS) involved in the biosynthesis of PG and CL belong to CDP-alcohol phosphotransferase and share overall amino acid sequence homology. However, it remains elusive whether PGPS and CLS are functionally distinct in vi...

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Cross-species complementation of bacterial- and eukaryotic-type cardiolipin synthases

The glycerophospholipid cardiolipin is a unique constituent of bacterial and mitochondrial membranes. It is involved in forming and stabilizing high molecular mass membrane protein complexes and in maintaining membrane architecture. Absence of cardiolipin leads to reduced efficiency of the electron transport chain, decreased membrane potential, and, ultimately, impaired respiratory metabolism. ...

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Cardiolipin puts the seal on ATP synthase.

ATP synthases are remarkable proteins that regenerate the molecular fuel for cellular processes in all domains of life (1). Embedded into the inner membranes of mitochondria, chloroplasts, and bacteria, FoF1-ATP synthase produces most of the cellular ATP, using ADP and inorganic phosphate as inputs (Fig. 1A). This thermodynamically unfavorable reaction is powered by a proton electrochemical gra...

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ژورنال

عنوان ژورنال: Proceedings of the National Academy of Sciences

سال: 2012

ISSN: 0027-8424,1091-6490

DOI: 10.1073/pnas.1121528109